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| Overview | More than 100 years ago it was known that there are elastic enzyme activity in the arteries and pancreas, but for a long time, it is considered to be trypsin, and pancreatic elastase was isolated from Balo and Banga until 1949. since then, it has been studied for its physiological action and pharmacological effect, and is clinically used for the treatment of hyperlipidemia, atherosclerosis and fatty liver. Japan was formally identified as a drug supply market in 1981. Elastase is the most striking feature is the dissolution of elastin, at the beginning people think that elastase is not a single enzyme, it contains elastic hydrolysis (elastic hydrolysis), viscous (hydrolysis) and lipid (hydrolysis) the role of a mixture of three enzymes, respectively, called elastase, elastic mucopolysaccharide enzyme and elastase. More recently, elastase is considered to contain two enzymes: elastase I, panereopolypeptide E (Ec. 3.4.4.7), commonly known as elastase, and elastase II, a newly discovered protease. Elastase is a serine-containing protease, which is composed of 240 amino acids and has a molecular weight of 25900. Its molecular peptide chain orientation and spatial configuration are similar to those of chymotrypsin, it does not contain a prosthetic group and metal ions, its active center depends on the specific three-dimensional structure, when its denaturation, configuration change, it causes inactivation. It is present in the pancreas of all animals as an inactive precursor (elastase). In the human aorta, skin, platelets and white blood cells also exist in the elastic enzyme activity of the material, in addition, some microorganisms and plants also have elastic enzyme activity of material. The elastase substrate has a wide specificity. It can dissolve most proteins, such as blood protein, albumin, casein, fibrin, soybean globulin, etc., in addition to dissolving elastin, but has no effect on hair keratin. In addition, it is also capable of decomposing many substrates for polypeptide synthesis. Elastase is involved in lipid metabolism in blood and arterial vessels in the human body. Animal experiments show that elastase has anti-arteriosclerosis effect and selective effect on denatured elastin, which can prevent the aorta, the formation of atherosclerotic plaque in coronary artery, enhance myocardial anti-hypoxia ability, protect ischemic myocardium, increase coronary flow, promote liver glycogen accumulation, prevent the formation of fatty liver, can prevent cholesterol synthesis and make it into bile acid and excretion, and significant decomposition of chylomicrons, promote lipid clearance from the blood without deposition in the arterial wall; can also reduce the urine beta 2-small globulin, inhibit the renal sphere basement membrane hypertrophy, prevent diabetic kidney disease; Can also reduce the total fat of fatty liver, inhibit the development of fatty liver; Can dissolve collagen, inhibition of lung tissue collagen increase, inhibition of bleomycin treatment side effects-pulmonary fibrosis; Can indirectly affect the activity of plasmin vasopressin; Can also be used for deformation of joint inflammation. In the clinical treatment of hyperlipidemia, diabetes and liver, lung disease. |
| pharmacological effects | an enzyme in this strain that dissolves elastin, pancreatic elastase is a polypeptide consisting of 240 amino acids, extracted from the pancreas of animals or produced by fermentation of microorganisms. This enzyme can prevent cholesterol synthesis in the body, and promote its conversion into bile acid, lower blood cholesterol, triglyceride, low density lipoprotein and very low density lipoprotein, increase high density lipoprotein, in addition, this product also promotes blood coagulation, strengthens the effect of uterine contractions. |
| uses | elastase preparations are mainly used to improve abnormal lipid metabolism in serum and tissues, promotes the dissimilation and excretion of cholesterol in the liver; Increases the activity of lipoproteinases, promotes the metabolism of neutral lipids and lipoproteins, especially very low density lipoproteins and low density lipoproteins. Can inhibit the occurrence of atherosclerosis and inhibit the formation of experimental liver fibrosis and fat. But occasional allergies and mild gastrointestinal symptoms
used to regulate blood lipids |
| production method | remove fire, wash fat, dry fresh or frozen pig pancreas, put it into a meat grinder, add 3 times the amount of acetone cooled to below 00C, under constant stirring at about 00C dehydration for 1H, centrifugation (waste acetone recovery treatment), separation of precipitation, 3 times the amount of cold acetone is added to the wet cake, and the same operation as above is carried out. The wet cake is dried in a vacuum drying oven and pulverized to obtain acetone dry powder. Cold sealed storage, standby. Crushed pig pancreas acetone (cold) → dehydration; Degreasing; Dry Acetone Powder extraction acetone dry powder add a certain amount of water according to the weight of Acetone Powder, make the weight volume ratio of 5%, under constant stirring, the extract is extracted at 20-250C for 2H, a small amount of talc is added during the filtration of the extract to help the filtration below 200C, and the cotton cloth is filtered naturally or by plate and frame pressure filtration to obtain a clear extract. Acetone Powder water: 200C → extract adsorption extract added a certain amount of distilled water diluted to 2-3 times, added to the pH 6.4 phosphate buffer (0.1mol/L) amberlite CG-50 resin m (raw material):m (resin) = 1: 2, under constant stirring, 20 ~ 25C adsorption 2H, the resin was rinsed with distilled water until the eluent was colorless to give an adsorbate. Extract solution Amberliet CG-50 resin → adsorbate elution 1 part of adsorption resin added 1.5 times ph9.3 ammonium chloride buffer (0.5mol/L), under constant stirring, elution 1H, separation resin, the eluent was adjusted to ph neutral with acetic acid (2mol/L) and filtered to obtain a clear eluent. Adsorbate Ph 9.3 buffer → elution eluent precipitate under-50C, add 3 times of acetone cooled to-50C to the eluent while stirring, continue stirring for 10min, at-50C, the patient was allowed to settle for several hours. Eluent acetone;-50C → precipitation precipitation dehydration, drying the precipitate was collected, washed 3 times with acetone, then washed 3 times with ether, and dried under vacuum to obtain elastase original powder. Acetone precipitation; Diethyl ether → dehydration; Dried elastase raw powder |